課程大綱

課程資訊

課程名稱

植物分子生物學
Plant Molecular Biology

開課學期

100-2

授課對象

生命科學院生化科技研究所

授課教師

黃鵬林

課號

HORT5014

課程識別碼

628 U1190

班次

學分

全/半年

半年

必/選修

必修

上課時間

星期五2,3,4(9:10~12:10)

上課地點

轉溫101

備註

A群組必修10學分課程之一。須先修過基礎植物分子生物學或分子生物學或類似課程。與杜宜殷合開
總人數上限：30人

Ceiba 課程網頁

http://ceiba.ntu.edu.tw/1002pmb

課程簡介影片

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課程概述

課程簡介:
一、內容
本課程涵蓋下列主題，著重於分子生物在植物生理、植物遺傳與育種上之應用，俾學習者具備研讀植物分子生物學論文之能力。
1. 高等植物之基因組大小及信使RNA複雜性
2. 模式植物---阿拉伯芥
3. 以傳統方法選殖植物基因
4. 以跳動因子標誌法選殖植物基因
5. 植物基因的構造
6. 高等植物之基因調控
7. 葉綠體基因及其調控
8. 不親和性之分子分析
9. 種子蛋白及其基因
10. 休眠與發芽之分子生物學
11. 生長激素控制之生長及基因表達
12. 植物逆境之分子生物學

二、教科書
General
1. Alberts B, Bray D, Lewis J, Raff M, Roberts & Watson JD (1994) Molecular Biology of the Cell, 3rd ed. Garland Publishing Inc, New York & London.
2. Dennis DT & Turpin DH (1990) Plant Physiology, Biochemistry and Molecular Biology. Longman Scientific & Technical. 529pp.
3. Grierson D & Covey SN (1998) Plant Molecular Biology, 2nd ed. Chapman and Hall, New York. 233pp.
4. Lewin B (1997) Genes VI. Oxford University Press, Oxford, New York & Tokyo.

Lab manual
1. Draper J, Scott R, Armitage P & Walden R (1988) Plant Genetic Transformation and Gene Expression - A Laboratory Manual. Blackwell Scientific Publication, Oxford, London, Edinburgh, Boston, Palo Alto & Melbourne.
2. Glover DM (1985) DNA Cloning - A Practical Approach, Vols I & II. IRL Press, London.
3. Glover DM (1989) DNA Cloning - A Practical Approach, Vol III. IRL Press, London.
4. Sambrook J, Fritsch EF & Maniatis T (1989) Molecular Cloning - A Laboratory Manual, 2nd ed. Vols 1, 2 & 3. Cold Spring Harbor Laboratory Press, New York.
5. Schuler MA & Zielinski RE ( 1989) Methods in Plant Molecular Biology. Academic Press Inc, San Diego.
6. Shaw CH (1988) Plant Molecular Biology - A Practical Approach. IRL Press, Oxford, Washington DC.
7. Weissbach A & Weissbach H (1988) Methods for Plant Molecular Biology. Academic Press Inc, San Diego.

三、成績評量方式
期終考試、期末考試與學期報告各佔三分之一。

四、預修課程
植物生理、生物化學。

課程目標

This course will focus on the application of molecular biology to plant physiology, plant genetics, and plant breeding.

課程要求

Plant physiology, Biochemistry, and Basic molecular biology

預期每週課後學習時數

Office Hours

另約時間

指定閱讀

參考書目

General

1. Alberts B, Bray D, Johnson A, Lewis J, Raff M, Roberts K & Walter P (1998) Essential Cell Biology - An introduction to the molecular biology of the cell. Garland Publishing Inc. New York & London.
2. Baxevanis AD & Ouellette BFF (2001) Bioinformatics – A Practical Guide to the Analysis of Genes and Proteins, 2nd ed. John Wiley & Sons, Inc. 470pp.
3. Buchanan BB, Gruissem W & Jones RL (2000) Biochemistry and Molecular
Biology of Plants. American Society of Plant Physiologists, Rockville, Maryland. 1367pp.
4. Cooper GM & Hausman RE (2004) The Cell: A Molecular Approach. 3rd ed. ASM Press, Washington, DC, USA.713pp.
5. Dennis DT & Turpin DH (1990) Plant Physiology, Biochemistry and Molecular
Biology. Longman Scientific & Technical. 529pp.
6. Gibson G & Muse SV (2002) A Primer of Genome Science. Sinauer Associates
Inc. 347pp.
7. Grierson D & Covey SN (1988) Plant Molecular Biology, 2nd ed. Chapman and
Hall, New York. 233pp.
8. Glick BR & Pasternak JJ (2003) Molecular Biotechnology, 3rd ed. ASM Press, Washington DC. 760pp.
9. Hannon GJ (2003) RNAi: A Guide to Gene Silencing. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, USA. 435pp.
10. Hawes C & Satiat-Jeunemaitre B (2001) Plant Cell Biology, 2nd ed. Oxford
University Press, New York. 338pp.
11. Heldt HW (1997) Plant Biochemistry and Molecular Biology. Oxford University Press, Oxford, New York & Tokyo. 522pp.
12. Howell SH (1998) Molecular Genetics of Plant Development. Cambridge University Press, Cambridge. 365pp.
13. Hughes MA (1996) Plant Molecular Genetics. Addison Wesley Longman Limited. Harlow. 236pp.
14. Hunt SP & Livesey FJ (2000) Functional Genomics. Oxford University Press, New York. 253pp.
15. Lea PJ & Leegood RC (1999) Plant Biochemistry and Molecular Biology. John
Wiley & Sons, Inc., New York. USA. 364pp.
16. Lewin B (2004) Genes VIII. Pearson Prentice Hall, Pearson Education, Inc. Upper Saddle River, NJ, USA. 1027pp.
17. Saito K, Dixon RA & Willmitzer L (2006) Plant Metabolomics. Biotechnology
in Agriculture and Forestry, Vol 57. Springer-Verlag Berlin Heidelberg. 347pp.
18. Thiellement H, Zivy M, Damerval C & Mechin V (2007) Plant Proteomics:
Methods and Protocols. Methods in Molecular Biology, Vol 335. Humana
Press Inc, Totowa, NJ. 399pp.
19. Vo-Dinh T (2005) Protein Nanotechnology, Protocols, Instrumentation,
and Applications. Methods in Molecular Biology, Vol 300. Humana Press Inc, Totowa, NJ. 463pp.
20. Westhoff P (1998) Molecular Plant Development. Oxford University Press,
Oxford, New York & Tokyo. 272pp.
21. Wu W, Welsh MJ, Kaufman PB & Zhang HH (2004) Gene Biotechnology. CRC
Press, Boca Raton, Florida. 523pp.

Lab manual

1. Draper J, Scott R, Armitage P & Walden R (1988) Plant Genetic Transformation and Gene Expression - A Laboratory Manual. Blackwell Scientific Publications, Oxford, London, Edinburgh, Boston, Palo Alto & Melbourne.
2. Gelvin SB & Schilperoort RA (1994) Plant Molecular Biology Manual, 2nd ed. Kluwer Academic Publishers, Dordrecht, Boston, London.
3. Gilmartin PM & Bowler C (2002) Molecular Plant Biology Volume One – A Practical Approach. Oxford University Press. 274pp.
4. Gilmartin PM & Bowler C (2002) Molecular Plant Biology Volume two – A Practical Approach. Oxford University Press. 332pp.
5. Micklos DA & Freyer GA (2003) DNA Science: A First Course. 2nd ed. Cold
Spring Harbor Laboratory Press, Cold Spring Harbor, New York, USA. 575pp.
6. Sambrook J, Russell DW & Sambrook J (2001) Molecular Cloning: A Laboratory Manual, 3rd ed. Vols 1, 2 & 3. Cold Spring Harbor Laboratory Press, New
York.
7. Schuler MA & Zielinski RE (1989) Methods in Plant Molecular Biology. Academic Press Inc, San Diego.
8. Shaw CH (1988) Plant Molecular Biology - A Practical Approach. IRL Press, Oxford, Washington DC.
9. Tagu D & Moussard C (2006) Techniques for Molecular Biology. Science Publishers, Enfield, NH, USA. 227pp.
10. Weigel D & Glazebrook J (2002) Arabidopsis. A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. 354pp.
11. Weissbach A & Weissbach H (1988) Methods for Plant Molecular Biology.
Academic Press Inc, San Diego.
12. Westermeier R & Naven T (2002) Proteomics in Practice – A Laboratory
Manual of Proteome Analysis. Wiley-VCH Verlag-GmbH, Weinheim. 316pp.
13. Wilson K & Walker J (2005) Principles and Techniques of Biochemistry
and Molecular Biology. Cambridge University Press, New York. 783pp.

評量方式
(僅供參考)

No.	項目	百分比	說明
1.	Midterm exam	33%
2.	Literature review	33%
3.	Term paper	34%

課程進度

週次	日期	單元主題
第1週	02/24	Introduction (Dr. Huang)
第2週	03/02	Genome organization and chromatin structures (Dr. Huang)
第3週	03/09	Arabidopsis as a model plant for functional genomics (Dr. Do)
第4週	03/16	Strategies of gene cloning and transposon tagging (Dr. Do)
第5週	03/23	Structural analysis of plant genes (Dr. Huang)
第6週	03/30	Regulation of plant gene expression (Dr. Huang)
第7週	04/06	Study Day
第8週	04/13	Molecular biology of chloroplasts and mitochondria in plants (Dr. Huang)
第9週	04/20	Midterm exam (Dr. Huang)
第10週	04/27	Flower development (Dr. Do)
第11週	05/04	Sexual incompatibility, seed development, and germination (Dr. Do)
第12週	05/11	Hormone action and regulation (I) (Dr. Huang)
第13週	05/18	Hormone action and regulation (II) (Dr. Huang)
第14週	05/25	Signal perception and transduction (I) (Dr. Huang)
第15週	06/01	Signal perception and transduction (II) (Dr. Huang)
第16週	06/08	Senescence and programmed cell death (I) (Dr. Huang)
第17週	06/15	Senescence and programmed cell death (II) (Dr. Huang)
第18週	06/22	Final exam (Dr. Huang)